|
|
 |
MD Assign: Three Generations of Plate Barcodes
SAMI® TIPS AND TRICKS
|
|
Christoph Krüll, Ph.D, Beckman Coulter
Question:
During my process, I want to create four 96-well daughter plates out of one 384-well plate. The daughter plates are then copied into assay plates within the same SAMI method. All plates are barcode labeled. I would like to log all three generations of barcodes in the assay plate's reader log file. Is this possible?
Answer:
This is possible. The following description assumes that SAMI version 3.0 or higher is used, and the user is familiar with the "MD Assign" module and the concept of transport properties.
We want to configure two MD Assign modules to collate all available barcodes on the final assay plate. Barcodes are considered Plate Properties can be logged together with e.g. reader data. Here we want to create three different plate properties to hold the three different barcodes of interest. We will use multiple configurations of a station. Compare the "Changing Parameters at Run Time" section in the SAMI user manual addendum for multiple configurations.
Setup:
Prepare a SAMI method as shown in Fig. 1. It is a good idea to place the MD Assign modules right after the liquid handling part, here a Biomek® FX. This is a logical place with both plates at the same time still on the liquid handler and simplifies scheduling of this action. Configure all icons to your settings; MD Assign icons just to the default settings for now. Right click on the first MD Assign module and select "Properties."
This will open a configuration window with the default settings of the MD Assign module as shown in Fig. 2. The default settings describe to read two different properties of the plate on Slot 1, the "Source" slot. The source plate's barcode will be copied to all plates on slots 2 - 6 (Destinations) to become the "Mother Barcode" property of these plates. Likewise, the source plate's "Name" is copied to become the destination's "Mother Name" property.
|
Fig. 1 SAMI Method Flowchart
Fig. 2 Default settings of the MD Assign
"Properties" dialog |
Following the first MD Assign in the SAMI method shown in Fig. 1, the "Grandmother" plate's Barcode and Name are copied to the "Mother" plate that carries the follow properties now:
| Name = |
plate name as defined in the "Mother" source node |
| Barcode = |
barcode printed on plate |
| Mother Barcode = |
barcode as printed on "Grandmother" plate |
| Mother Name = |
plate name as defined in the "Grandmother" source node |
Now, right-click on the second (!) MD Assign and select "Properties." We want to change the default settings to copy the "Grandmother" barcode to the "Daughter" in addition to the "Mother" barcode. Leave the first line unchanged, the source plate's ("Mother") barcode will be copied to the "Mother Barcode" property on the destination plate ("Daughter"). We are not interested in the source plate's name and thus we will use the existing "Mother Name" property to hold the "Grandmother" barcode.
| Simply select the field "Property" in the second line of the "Properties" configuration dialog and type "Mother Barcode" to overwrite the default "Name" as shown in Fig. 3. Be careful with correct spelling and confirm the change with "OK." Please ensure that the check box "Ignore Future Changes by the Module" is checked as shown in Fig. 3. Run time, the modified MD Assign will now read the "Mother Barcode" property of the source plate ("Mother") to copy it to the "Mother Name" property of the destination plate ("Daughter"). |
Fig. 3 Configure Properties to report
"Grandmother" barcode |
Following this second MD Assign in the SAMI method shown in Fig. 1, the "Daughter" plate carries the following properties now:
| Name = |
plate name as defined in the "Daughter" source node |
| Barcode = |
barcode printed on plate |
| Mother Barcode = |
barcode as printed on "Mother" plate |
| Mother Name = |
barcode as printed on "Grandmother" plate |
Now simply configure your reader log file to include all four properties as listed above.
More SAMI® Tips and Tricks
SAMI® Log File Sorting
Horizontal Loading of Carousel and CO2 Incubator
One Log File Per Plate
Print & Apply: Printing a 'Virtual' Barcode
Additional Information:
* All trademarks are the property of their respective owners. Where applicable,
the PCR process is covered by patents owned by Roche Molecular Systems, Inc.,
and F. Hoffmann-LaRoche, Ltd.
For Research Use Only; not for use in diagnostic procedures.
For comments or questions about T3 Update, please contact the T3 Editor.
Email this page to a colleague
|
