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A CE-based system for the separation and analysis of carbohydrates. The system is ideal for both oligosaccharide profiling and monosaccharide analysis. System includes a P/ACE MDQ configured with a laser-induced fluorescence detector, 488 nm argon ion laser module, an ambient sample storage module, and 32 Karat™ Software configured on an IBM personal computer. Installation Qualification, Operation Qualification 1 (OQ1) and documentation to aid in software validation is also included.
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Product Features
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Oligosaccharide Profiling
The distribution of glycans released from a glycoprotein yields a fingerprint used in product identification and in monitoring production processes. CE-LIF provides rapid analysis of released glycans, with separation times typically less than 15 minutes. Extraordinary resolution is achieved, including some positional isomers. Laser-induced fluorescence provides high sensitivity, enabling you to simply "dilute and shoot" samples with complex matrices.
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Identity by Immunoassay
Immunoassays are commonly used in biotechnology for the detection and quantification of host cell contaminants. The CE-LIF format eliminates antigen immobilization and avoids many solid phase-associated problems. Since laser-induced fluorescence detection is used, nonlabeled proteins and peptides that do not interact with the complex are not detected, giving clean baselines and easily interpreted electropherograms.
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Monosaccharide Analysis
The analysis of monosaccharide composition can provide valuable
information during the initial characterization of a glycoprotein. A
clear advantage of our CE-based approach is in the specificity of the
assay. The reductive amination and open-tube format allow this analysis
to be performed directly from the glycoprotein hydrolysate--no need for
posthydrolysis cleanup. With LIF and APTS derivatization, each sugar
yields the same detector response, such that their relative quantities
can be directly compared.
CE separation of (A) the mild acid hydrolysis product of fetuin after
aldolase treatment. (B) the strong acid hydrolysis product after
reacetylation and (C) monosaccharide standards. Capillary: 27 cm x 25 um
bare fused silica; Buffer: 240 mM borate (pH 9.0); Field strength: 740
Volts/cm; Injection: 0.5 psi for 5; Detection: Laser-induced
fluorescence (excitation at 488 nm, emission at 520 nm).
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Solutions for the Analysis of Macromolecules
This CD-ROM tutorial provides seminars on the development of solution-based assays for nucleic acid, protein and carbohydrate analyses. Animation describing these analytical processes is shown, along with practical demonstrations on the implementation of these assays. Our focus is to provide you with tools that improve productivity by cutting through the complexity with simple, bold analytical solutions.
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