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Employment of robust and simple characterization tools is required in the development and commercialization of biotherapeutics. Capillary electrophoresis technology is rapidly migrating into quality control environments. A large number of industrial biotechnology laboratories utilize the ProteomeLab™ PA 800 Protein Characterization System to analyze therapeutic proteins, and have been successful in the characterization of monoclonal antibodies.
Beckman Coulter is introducing a series of performance enhancements for the PA 800 system. These enhancements provide users a robust and easy-to-use characterization tool for the size separation of proteins, the analysis of carbohydrates and the determination of protein pI. Innovations have been made in hardware, software and operational supplies.
The ProteomeLab PA 800 Protein Characterization System represents a standardized technology for analysis of purity, heterogeneity and identity of therapeutic biologics. Through the technical enhancement of key components on the existing platform, we have advanced the capabilities of the PA 800 system, making it easier to operate and more robust in the applications for which it is being used.
Learn more about PA 800 applications and software.
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Product Features
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A Robust Solution for Demanding Research Requirements
The system offers dependable, accurate determination of protein purity, heterogeneity, and identity. To create it, Beckman Coulter collaborated with biopharmaceutical development and QC groups experienced in the routine use of capillary electrophoresis for protein characterization.
Automated Sample Introduction
The system offers fully automated methods and extended sample-handling capability for walk-away operation. Sampling can be performed using 1.8 ml universal vials, 96-well plates and micro vials. Precision-molded polymethylpentene universal vials accommodate run buffer, sample and micro vials.
Sample Temperature Control
Sample temperature control maintains molecular stability when working with temperature-labile protein species. The sample temperature can be maintained between 4° - 60°C.
Temperature Control of the Capillary
Efficient separations in CE rely on precise regulation of the capillary temperature to manage Joule heating within the capillary. Proper temperature control plays an important role in the repeatability of SDS-gel, cIEF and carbohydrate analysis.
The system uses recirculating liquid coolant to provide effective heat dissipation when performing assays on the system. Capillaries are housed in patented cartridges, facilitating both temperature control and easy exchange of capillary dimensions and surfaces (US Patent #5198091; see application bulletin T1823ab). Capillary temperature can be regulated between 15° - 60°C.
Multiple Modes of Sample Introduction and Separation
The system offers electrokinetic, pressure and vacuum injection of samples. Additionally, injection from either end of the capillary allows both ultra-fast and high-resolution analysis. Separations can be adjusted by varying voltage, current, pressure and vacuum. The combination of voltage and pressure in the SDS-gel assay ensures the gel buffer stays free of air bubbles which can be generated from gel outgassing.
- Photodiode array detection lets you perform high-sensitivity analyses across a broad range of wavelengths. Photodiode array detection between 190 and 600 nm allows for baseline subtraction and spectral wavelength analyses.
- The system UV/Vis Detection Module provides absorbance spectroscopy in the UV-visible region. Commonly used exclusion filters at 200 nm, 214 nm, 254 nm and 280 nm are provided to increase analyte specificity.
- The 488 nm solid state laser module provides robust fluorescence technology in a quiet, energy-efficient and compact design.
Variable Pressure and Vacuum
The system operates with all common rinsing protocols, regulating them with a pressure-handling capability of -5 to 100 p.s.i. Capillary conditioning is accomplished by moving specific volumes of electrolytes, gels, regenerants and cleaning solutions through the capillary. Gel buffers are quickly and efficiently pumped into the capillary.
Versatile Modular Detection Capability
Each system offers precise, real-time analysis for a variety of assays, because it integrates UV, photodiode array and LIF detection capabilities in one unit. UV detection is important when using photosensitive capillary surfaces. Photodiode array detection between 190 and 600 nm allows for baseline subtraction and spectral wavelength analyses. A 488 nm solid-state laser and laser-induced fluorescence (LIF) detector permits high-sensitivity analysis of labeled molecular species.
CE-MS Ready
Capillary electrophoresis separation coupled to mass spectrometry (MS) combines the high-resolution separation of CE and the high-sensitivity mass determination of MS. The system accommodates direct MS connection through the right-side access panel. Capillary temperature control is retained.
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