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DU® 800 UV/Vis Spectrophotometer
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The DU 800 UV/Visible Spectrophotometer is a scanning instrument with a bandwith of less than or equal to 1.8 nm and a wavelength range from 190 to 1100 nm.
All instruments include: DU 800 Windows 2000-Based System Control, RediScan™, RediRead™, Wavelength Scan, Fixed Wavelength (up to 12 wavelengths), Kinetics/Time (12 reactions), Single Component Analysis, Nucleic Acid Analysis (Ratios), Performance Validation, Full Support of 21 CFR Part 11 (Electronic Records; Electronic Signature), and Start-Up Diagnostics.
Additional software applications are available and may be added to the system. (See Software Options).
The DU 800 is a customer-installed spectrophotometer and System Installation should be ordered separately (except when service installation is applicable).
DU 800 UV/Vis Spectrophotometers are available with and without PC and Display. When ordered with the Beckman Coulter-supplied PC System, the DU 800 System and Applications Software is pre-installed and the system is ready to use.
When the PC is not ordered with the spectrophotometer, it is the responsibility of the customer to furnish a compatible PC with monitor and to supply and install Microsoft* Windows* 2000 as well as the DU 800 System and Applicaitons Software, which is included with the spectrophotometer.
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DU 800 Spectrophotometers
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The following instrument systems with and without transports (installed) and with and without IBM PC Systems are available.
For Single Sample Analysis
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| Part No. |
Description |
| 512800
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DU 800 UV/Vis Spectrophotometer
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| 512802
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DU 800 UV/Vis Spectrophotometer with IBM PC and Integrated 15" Flat-Panel Display
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For Multi-Sample Analysis
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| Part No. |
Description |
| 512804
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DU 800 UV/Vis Spectrophotometer with Standard Transport
Installed (P/N 517522)
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| 512806
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DU 800 UV/Vis Spectrophotometer with Standard Transport
Installed (P/N 517522), IBM PC and Integrated 15" Flat-Panel Display
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| 512809
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DU 800 UV/Vis Spectrophotometer with High Performance Transport, Installed (P/N 517040)
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| 512808
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DU 800 UV/Vis Spectrophotometer with High Performance Transport
Installed (P/N 517040), IBM PC and Integrated 15" Flat-Panel Display
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DU 800 Spectrophotometer Application Packages
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All packages come complete with a DU 800 UV/Vis spectrophotometer along with its standard features and software. See the following package descriptions for additional software options, accessories and IBM PC systems included in each.
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| Part No. |
Description |
| 512902
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DU® 800 Concentration Package - without IBM PC System
Includes: DU 800 UV/Vis Spectrophotometer, Single Cell Holder, 4.2 mL UV Silica Cuvette
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| 512903
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DU® 800 Concentration Package - with IBM PC System
Includes: DU 800 UV/Vis Spectrophotometer, Single Cell Holder, 4.2 mL UV Silica Cuvette, and IBM PC with integrated 15" flat-panel display.
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| 512900
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DU® 800 Life Science Package - without IBM PC System
Includes: DU 800 UV/Vis Spectrophotometer, Protein Assay Analysis Software Option, Nucleic Acid Analysis II Software Option, Single Cell Holder, Single Cell Holder for 50 µL Microcell, 50 µL Microcell (Qty. 2).
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| 512901
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DU® 800 Life Science Package - with IBM PC System
Includes: DU 800 UV/Vis Spectrophotometer, Protein Assay Analysis Software Option, Nucleic Acid Analysis II Software Option, Single Cell Holder, Single Cell Holder for 50 µL Microcell, 50 µL Microcell (Qty. 2), and IBM PC with integrated 15" flat-panel display.
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| 512904
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DU® 800 Kinetics Package - without IBM PC System
Includes: DU 800 UV/Vis Spectrophotometer, Enzyme Mechanism Software Option, Protein Assay Analysis Software Option, Standard Transport, Auto 6 Cell Holder (water-regulated).
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| 512905
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DU® 800 Kinetics Package - with IBM PC System
Includes: DU 800 UV/Vis Spectrophotometer, Enzyme Mechanism Software Option, Protein Assay Analysis Software Option, Standard Transport, Auto 6 Cell Holder (water regulated), and IBM PC with integrated 15" flat-panel display.
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| 390391
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DU® 800 High-Performance Kinetics Package - without IBM PC System
Includes: DU 800 UV/Vis Spectrophotometer, Enzyme Mechanism Software Option, Protein Assay Analysis Software Option, High-Performance Transport, Auto 6 Cell Holder (Peltier controlled). NOTE: The appropriate Peltier Temperature Controller is required when temperature control is desired.
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| 390392
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DU® 800 High-Performance Kinetics Package - with IBM PC System
Includes: DU 800 UV/Vis Spectrophotometer, Enzyme Mechanism Software Option, Protein Assay Analysis Software Option, High-Performance Transport, Auto 6 Cell Holder (Peltier controlled), and IBM PC with integrated 15" flat-panel display. NOTE: The appropriate Peltier Temperature Controller is required when temperature control is desired.
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| A49423
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DU800 Life Science Spectrophotometer and nanoVette package - without IBM PC
Includes: DU800 Life Science Spectrophotometer p/n A512900 and nanoVette with 0.2mm pathlength p/n A44097
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DU 800 Software Options
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| Part No. |
Description |
| 512984
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Wavelength Scan II
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| 512980
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Nucleic Acid Analysis II
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| 512981
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Protein Assay Analysis
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| 512982
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Enzyme Mechanism
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| 512983
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Enzyme Activity
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System Installation
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| Part No. |
Description |
| 517042
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DU 600/800 System Installation
Installation and training on DU 800 by certified Beckman Coulter Service Engineer. Includes training on the basic functionality of the instrument and its applications at the time of installation for a maximum of 4 hours.
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Experimental Microvolume Tm Analysis Accessory
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Experimentally determines the melting point temperature of up to six 325 µL DNA samples with denaturation and renaturation profiles within a temperature range from 0 to 110°C. Includes High Performance Peltier Temperature Controller, Micro Auto 6 Tm Cell Holder, six 325 µL Tm Microcells with Stopper, and the Tm Analysis Software Option.
Requires High-Performance Transport (P/N 517040).
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| Part No. |
Description |
| 390263
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Experimental Microvolume Tm Analysis Accessory
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Replacement Parts for Experimental Microvolume Tm Analysis Accessory
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| Part No. |
Description |
| 523880
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325 µL Tm Microcell with Stopper (one cell)
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| 523878
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325 µL Tm Microcell with Stopper (package of 6 cells)
Included with Experimental Microvolume Tm Analysis Accessory (P/N 390263 and 390264).
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| 517449
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Micro Auto 6 Tm Cell Holder
Included with Experimental Microvolume Tm Analysis Accessory (P/N 390263 and 390264).
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| 512985
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Tm Analysis Software
Included with Experimental Microvolume Tm Analysis Accessory (P/N 390263 and 390264).
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Regulatory & Compliance
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For assurance to regulatory agencies that new instrumentation has been certified in your laboratory before generating any test results.
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| Part No. |
Description |
| 512941
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DU® 800 Software Validation Pack
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| 512986
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DU® Series 800 Certification (OQ 3)
For additional coverage to a support agreement or for operational qualification during installation.
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Data Output Options
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| Part No. |
Description |
| 977510
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HP DeskJet Color ink-jet printer (100 to 240V)
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| 970207-01
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Parallel Printer Cable, Bi-directional
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Transports & Temperature Control
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| Part No. |
Description |
| 517522
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Standard Transport
For automatic positioning and movement of samples in DU® 800. Compatible with all ambient & water temperature-regulated accessories.
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| 517040
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High-Performance Transport
For automatic positioning/movement of samples in DU® 800. Compatible with all ambient and water- or Peltier temperature-regulated accessories.
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| 517057
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Peltier Temperature Controller, 120 V
For temperature control in the range of 20° to 40°C +0.2°C.
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| 517450
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High Performance Peltier Temperature Controller, 120 V
For experimental Tm temperature control from 0 to 110°C. Compatible with all Peltier temperature-controlled cell holders.
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Single Cell Holders
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The following accessories are placed on the static mount, provided with the DU 800 Cat. Nos. 512800, 512801, 512802, 512901, 512900, 512903, 512902. They can also be used with a transport (P/N 517040 or 517522).
Click here for more information on the Turbidity Cell Holder (PDF Format)
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| Part No. |
Description |
| 594439
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Single Cell Holder, Unheated.
Holds one standard cell, Semi-Microcell (2 or 4 mm pathwidth) with 10 mm pathlength and the 5 µL Ultra-Microcell or flow cell. Not for use with the 50 µL Microcell.
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| 523407
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Single Cell Holder, Water-regulated temperature control.
Includes quick disconnects and tubing for installation to water source. Holds one standard cell, Semi-Microcell (2 or 4 mm pathwidth) with 10 mm pathlength and the 5 µL Ultra-Microcell, or flow cell.
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| 523403
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Single Cell Holder, Peltier temperature control.
Holds one standard cell, Semi-Microcell or Flow Cell. Requires Peltier Temperature Controller (P/N 517057 or 517450) and Transport (P/N 517040).
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| 523382
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Single Cell Holder for 50 µL Microcell
Unheated, holds a single 50 µL Microcell assuring proper alignment of the sample and permits easy lifting of the cell from the instrument.
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| 517151
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Turbidity Cell Holder, Unheated.
For DU® Series 800 to accurately measure cell growth. Holds one standard cell or Semi-Microcell (2 or 4 mm pathwidth) with 10 mm pathlength and the 5 µL Ultra-Microcell.
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| 517037
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Rectangular Cell Holder, Unheated.
Holds one cell 5 mm to 50 mm long.
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| 517038
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Cylindrical Cell Holder, Unheated.
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Multi-Position Cell Holders
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The following accessories require the transport (P/N 517040 or 517522).
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| Part No. |
Description |
| 523430
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Auto 6 Cell Holder, Unheated, includes removable cell basket.
Holds up to six standard cells or Semi-Microcells (2 or 4 mm pathwidth) with 10 mm pathlength.
Not for use with the 5 µL Ultra-Microcell or 50 µL Microcell.
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| 523415
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Auto 6 Cell Holder, Water-regulated temperature control.
Includes removable cell basket, quick disconnects and tubing for installation to water source. Holds up to six standard cells or Semi-Microcells (2 or 4 mm pathwidth) with 10 mm pathlength.
Not for use with the 5 µL Ultra-Microcell or 50 µL Microcell.
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| 523409
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Auto 6 Cell Holder, Peltier temperature control.
Requires Peltier Temperature Controller (P/N 517057 or 517450) and Transport (P/N 517040). Holds up to six standard cells or Semi-Microcells (2 or 4 mm pathwidth) with 10 mm pathlength.
Not for use with the 5 µL Ultra-Microcell or 50 µL Microcell.
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| 517518
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Auto 6 Cell Holder w/ Stirring, 115V
Water-regulated temperature control and magnetic stirring of all six cuvettes. Includes removable cell basket, quick disconnects and tubing for installation to water source. Holds up to six standard cells or Semi-Microcells (2 or 4 mm pathlength).
Not for use with the 5 µL Ultra-Microcell or 50 µL Microcell.
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| 517519
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Auto 6 Cell Holder w/ Stirring, 230V
Water-regulated temperature control and magnetic stirring of all six cuvettes. Includes removable cell basket, quick disconnects and tubing for installation to water source. Holds up to six standard cells or Semi-Microcells (2 or 4 mm pathlength).
Not for use with the 5 µL Ultra-Microcell or 50 µL Microcell.
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| 523386
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Micro-Auto 6 Cell Holder
Unheated, for six 50 µL cells.
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| 517039
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Auto 7 Cell Holder
For up to six samples and reference or blank.
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| 517053
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Auto 8 Cell Holder
For up to six samples and reference and/or blank.
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| 514254
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Micro-Auto 12 Cell Holder, Water-regulated temperature control.
For up to twelve 100 µL samples. Holds up to three 4-position Multi-Microcells (P/N 514255). Includes quick disconnects and tubing for installation to water source.
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Sipper and Batch Sampler
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| Part No. |
Description |
| 514242
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Sipper Accessory
Automated delivery of a sample to a flow cell. Requires desired flow cell, tubing kit and cell holder.
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| 514253
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Flow Cell and Tubing Kit
Includes 80 µL unheated flow cell (P/N 599925)
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| 514256
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Micro-Sipper Cell Accessory
Includes 50 µL Aspiratable Microcell (P/N 514758), Tubing Kit (P/N 514282) and retaining clip. Requires cell holder for 50 µL cell (P/N 523382 or P/N 523386). Requires Sipper Accessory (P/N 514242).
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| 512814
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Batch Sampler, 115 - 240 V
For automated analysis of up to 114 samples. Includes interface and cables. Requires sipper accessory, cell holder, flow cell and tubing kit.
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Sipper Supplies
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| Part No. |
Description |
| 599925
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80 µL Flow Cell, Threaded Fittings, 10 mm Pathlength.
For Sipper Sampling Module.
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| 514758
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Micro-Sipper Cell, 50 µL
Cell replacement for aspiratable Micro-Sipper Accessory.
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| 514745
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Sipper/Batch Tubing (Unheated)
Three sets of inlet and outlet tubing for unheated sipper (threaded) for Beckman Coulter sampler.
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| 514282
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Micro-Sipper Tubing Kit
For use with Aspiratable Microcell.
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| 651731
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Peristaltic Pump Tubing (quantity 1)
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| 586656
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Waste Bottle
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nanoVette Microliter Measurement Cell
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Allows your Beckman Coulter spectrophotometer to work with sample sizes as small as 0.7 µL. Perfect for DNA and Protein applications where small sample size is critical, the nanoVette provides the additional advantage of not requiring dilution of the sample. The same size as a standard cuvette, installs and removes simply into the DU spectrophotometer and measures dsDNA concentrations up to 5,000 ng/µL.
Click here for more information on the nanoVette
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| Part No. |
Description |
| A44097
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nanoVette with 0.2 mm pathlength lid
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| A44098
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nanoVette with 1.0 mm pathlength lid
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| A44100
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nanoVette with 0.2 and 1.0 mm pathlength lid
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| A44102
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0.2 mm pathlength lid for nanoVette
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| A44103
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1.0 mm pathlength lid for nanoVette
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Microcells and Ultra-Microcell
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Our unique microcells with their specially designed cell holders enable the user to achieve excellent spectrophotometric precision and accuracy with only 50 µL or just 5 µL of sample.
Requires appropriate cell holder (see "Single Cell Holders," earlier).
Click here for more information on the Ultra-Microcell (PDF Format)
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| Part No. |
Description |
| 523270
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Microcell, 50 µL (quantity 1)
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| 523450
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Microcell, 50 µL (quantity 2)
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| 523451
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Microcell, 50 µL (quantity 4)
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| 523452
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Microcell, 50 µL (quantity 6)
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| 514261
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Ultra-Microcell, 5 µL
With tube of 100 quartz capillaries (manufactured by General Atomics) (quantity 1).
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| 514255
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Multi-Microcell, 4-Position 100 µL Cell
Not available for DU® 640B.
Up to three Multi-Microcells (12 samples) can be placed in the Micro-Auto 12 Cell Holder (P/N 514254).
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Replacement Parts and Supplies
Source Lamps
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| Part No. |
Description |
| 514259
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Tungsten-Halogen Lamp
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| 514366
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Deuterium Lamp
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Sampling Cell Supplies
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| Part No. |
Description |
| 594588
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Cell Holder Spring Replacement
For multi-position cell holders. This spring holds the cells in the cell basket.
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| 590122
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Cell Basket, 6 Sample
For use with multi-position cell holders having removable cell baskets.
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| 897997
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Cell Holder, 1-mm
For use with 1 mm cells in a 10 mm cell holder.
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| 514262
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Replacement Quartz Capillaries
Tube of 100 quartz capillaries for Ultra-Microcell.
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| 517456
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Replacement Seals for Tm Cell Holder (package of 6 seals)
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Performance Validation
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| Part No. |
Description |
| 517698
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Holmium Oxide Filter
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Manuals and Other
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| Part No. |
Description |
| 390460
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DU® 800 System and Applications Software (CD-ROM)
Included with instrument.
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| 512860
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DU® 800 Installation and Operating Instructions
Included with instrument.
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Cuvettes and Cells
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Beckman Coulter provides a wide variety of cuvettes and flow cells for use on the DU® Series Spectrophotometers. Please refer to the charts below for part numbers.
Rectangular, Open Top
| Part No. |
Path
Length
(mm) |
Total
Volume
(mL) |
Min.
Volume*
(mL) |
Path
Width
(mm) |
Window
Material |
No.
of
Cells |
Cell |
| 580011 |
5 |
2.1 |
0.25 |
10 |
UV Silica |
1 |
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| 886506 |
10 |
4.2 |
0.5 |
10 |
Optical Gla |
1 |
| 75152 |
10 |
4.2 |
0.5 |
10 |
Optical Gla |
4 |
| 596492 |
10 |
4.2 |
0.5 |
10 |
Optical Gla |
6 |
| 580012 |
10 |
4.2 |
0.5 |
10 |
UV Silica |
1 |
| 596493 |
10 |
4.2 |
0.5 |
10 |
UV Silica |
6 |
| 886510 |
50 |
22.5 |
2.5 |
10 |
Optical Gla |
1 |
Rectangular, Stoppered
| Part No. |
Path
Length
(mm) |
Total
Volume
(mL) |
Min.
Volume*
(mL) |
Path
Width
(mm) |
Window
Material |
No.
of
Cells |
Cell |
| 580015 |
10 |
3.8 |
0.5 |
10 |
UV Silica |
1 |
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| 596494 |
10 |
3.8 |
0.5 |
10 |
UV Silica |
6 |
Semi-Microcell, Masked
| Part No. |
Path
Length
(mm) |
Total
Volume
(mL) |
Min.
Volume*
(mL) |
Path
Width
(mm) |
Window
Material |
No.
of
Cells |
Cell |
| 533041 |
10 |
1.2 |
0.150 |
2.0 |
UV Silica |
2 |
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| 533040 |
10 |
1.2 |
0.150 |
2.0 |
UV Silica |
4 |
| 533043 |
10 |
2.0 |
0.250 |
4.0+ |
UV Silica |
2 |
| 533042 |
10 |
2.0 |
0.250 |
4.0+ |
UV Silica |
4 |
| 517056 |
10 |
2.0 |
0.250 |
4.0+ |
UV Silica |
6 |
Semi-Microcell, Stoppered
| Part No. |
Path
Length
(mm) |
Total
Volume
(mL) |
Min.
Volume*
(mL) |
Path
Width
(mm) |
Window
Material |
No.
of
Cells |
Cell |
| 886562 |
10 |
0.8 |
0.100 |
2.0 |
UV Silica |
1 |
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50 µL Microcell, Masked
| Part No. |
Path
Length
(mm) |
Total
Volume
(mL) |
Min.
Volume*
(mL) |
Path
Width
(mm) |
Window
Material |
No.
of
Cells |
Cell |
| 523270 |
10 |
0.1 |
0.050 |
2.0 |
UV Silica |
1 |
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| 523450 |
10 |
0.1 |
0.050 |
2.0 |
UV Silica |
2 |
| 523451 |
10 |
0.1 |
0.050 |
2.0 |
UV Silica |
4 |
| 523452 |
10 |
0.1 |
0.050 |
2.0 |
UV Silica |
6 |
Multi-Microcell, Masked
| Part No. |
Path
Length
(mm) |
Total
Volume
(mL) |
Min.
Volume*
(mL) |
Path
Width
(mm) |
Window
Material |
No.
of
Cells |
Cell |
| 514255 |
10 |
0.35 |
0.100 |
3.0 |
UV Silica |
1 |
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5 µL Ultra-Microcell and Replacement Capillaries
| Part No. |
Path
Length
(mm) |
Total
Volume
(mL) |
Min.
Volume*
(mL) |
Path
Width
(mm) |
Window
Material |
No.
of
Cells |
Cell |
| 514261 |
0.5 |
0.007 |
0.005 |
0.5 |
UV Silica |
1 |
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| 514262 |
0.5 |
0.007 |
0.005 |
0.5 |
UV Silica |
100 |
325 µL Tm Microcell, ** Stoppered
| Part No. |
Path
Length
(mm) |
Total
Volume
(mL) |
Min.
Volume*
(mL) |
Path
Width
(mm) |
Window
Material |
No.
of
Cells |
Cell |
| 523880 |
10 |
0.445 |
0.1 |
3 |
UV Silica |
1 |
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| 523878 |
10 |
0.445 |
0.1 |
3 |
UV Silica |
6 |
325 µL Tm Microcell, **
| Part No. |
Path
Length
(mm) |
Total
Volume
(mL) |
Min.
Volume*
(mL) |
Path
Width
(mm) |
Window
Material |
No.
of
Cells |
Cell |
| 517481 |
10 |
0.445 |
0.1 |
3 |
UV Silica |
1 |
|
| 517475 |
10 |
0.445 |
0.1 |
3 |
UV Silica |
6 |
Gel Cuvette
| Part No. |
Path
Length
(mm) |
Total
Volume
(mL) |
Min.
Volume*
(mL) |
Path
Width
(mm) |
Window
Material |
No.
of
Cells |
Cell |
| 198649 |
7 |
-- |
-- |
100 |
UV Silica |
1 |
|
* Requires aperture slit of 2 mm height.
** Requires Micro Auto 6 Tm Cell Holder, which is part of the Micro Tm
Analysis Accessory.
+ These pathwidths are recommended with six-position auto-transports.
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