Plasmid Purification using Wizard® SV 96

Related Product Information

Plasmid Purification using Wizard® SV 96 and Wizard® SV 9600 Plasmid DNA Purification Systems

The Wizard® SV 96 and SV 9600 Plasmid DNA Purification Systems are designed to be adapted for use with automated purification instruments. These systems allow the purification of plasmid suitable for the use in automated fluorescent sequencing as well as other standard molecular biology applications, including restriction enzyme digestions.

In a high-throughput application, elution of DNA samples into small volume 96 well microtiter plates is the preferred method of collection and storage of DNA samples. This can be a problem when using certain 96-well plasmid purification systems. A unique plate design has been used with the Wizard® SV 96 System to minimize spraying. The larger aperture and increased length of the elution ports allows eluted DNA to be collected in standard 96-well microtiter plates.

Wizard® SV 96 plate tips

commercially available 96 well plate

Plate tip design comparison. Photographs show increased tip aperture and absence of skirt for the Wizard® SV 96 plate tips (top) versus the tips of a second commercially available 96 well plate (bottom).

The SV 96 system contains all the reagents necessary for purification of plasmid DNA, including:

Picture of a Promega Wizard SV 96 Plasmid Purification kit

96 Well Culture Plates-- allowing growth of up to 96 x 1.5ml cultures at a time
Lysate Clearing Plates-- providing a rapid alternative to centrifugation for removal of cellular material from bacterial lysates without centrifugation
DNA Binding Plates-- purifying high quality plasmid DNA

The Wizard® SV 9600 System contains Clearing and Binding Plates as well as reagents to purify 9600 (100 x 96) minipreps on a robotic workstation in the most high-throughput setting.

Beckman Coulter-Promega Wizard SV 96 and SV 9600 Plasmid Purification Systems and Related Products

Part No.	Description
725200	Wizard SV 9600 Plasmid DNA Purification System (100x96)
725211	Wizard SV 96 Wash Solution (370 mL)
725212	Wizard SV 96 Neutralization Solution (900 mL)
725213	Wizard SV 96 Lysate Clearing Plate (100 plates)
725214	Wizard SV 96 Binding Plate (100 plates)
725215	Wizard SV 96 Cell Resuspension Solution (800 mL)
725216	Wizard SV 96 Cell Lysis Solution (800 mL)
725230	Water, Nuclease Free (150 mL)
608133	Wizard SV 96 Plasmid DNA Purification System (1x96)
608134	Wizard SV 96 Plasmid DNA Purification System (5x96)
608136	Wizard SV 96 DNA Binding Plates (10 plates)
608137	Wizard SV 96 Lysate Clearing Plates (10 plates)
608138	Wizard SV 96 Cell Resuspension Solution (500 mL)
608139	Wizard SV 96 Cell Lysis Solution (500 mL)
608140	Wizard SV 96 Neutralization Solution (100 mL)
608141	Wizard SV 96 Wash Solution (185 mL)

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